added annotation part

16S_workflow.sh

@@ -48,7 +48,10 @@ soft/vsearch --uchime_denovo fasta/prefix.fasta --nonchimeras fasta/prefix_nochi
 
 # CLUSTERING
 otu=">OTU_"
-vsearch --cluster_fast fasta/prefix_nochim.fasta --id 0.97 --centroids otu/centroids.fasta --relabel $otu
+vsearch --cluster_fast fasta/prefix_nochim.fasta --id 0.97 --centroids otu/otu.fasta --relabel $otu
 
 # ABONDANCE
-vsearch --usearch_global fasta/amplicon.fasta --db otu/clusters.fasta --id 0.97 --otutabout otu/abondance_table.txt
+vsearch --usearch_global fasta/amplicon.fasta --db otu/otu.fasta --id 0.97 --otutabout otu/abondance_table.txt
+
+# ANNOTATION
+soft/vsearch --usearch_global otu/otu.fasta --db databases/mock_16S_18S.fasta --id 0.97 --top_hits_only --userfields query+target --userout otu/annotation.txt