commit 069e99e46bcbb4b4699d781081f831a24402e811 Author: Amine GHOZLANE Date: Thu Oct 17 21:47:21 2019 +0200 First commit diff --git a/.gitignore b/.gitignore new file mode 100644 index 0000000..162f6c0 --- /dev/null +++ b/.gitignore @@ -0,0 +1,2 @@ +__py_cache__ +*.pyc diff --git a/data/eva71.fna b/data/eva71.fna new file mode 100644 index 0000000..dd5d6fe --- /dev/null +++ b/data/eva71.fna @@ -0,0 +1,151 @@ +>EVA71_BrCr_U22521 EVA71 +TTAAAACAGCTGTGGGTTGTCACCCACCCACAGGGTCCACTGGGCGCT +AGTACACTGGTATCTCGGTACCTTTGTACGCCTGTTTTATACCCCCTCCC +TGATTTGCAACTTAGAAGCAACGCAAACCAGATCAATAGTAGGT +GTGACATACCAGTCGCATCTTGATCAAGCACTTCTGTATCCCCGGACCGA +GTATCAATAGACTGTGCACACGGTTGAAGGAGAAAACGTCCGTTACCCGG +CTAACTACTTCGAGAAGCCTAGTAACGCCATTGAAGTTGCAGAGTGTTTC +GCTCAGCACTCCCCCCGTGTAGATCAGGTCGATGAGTCACCGCATTC +CCCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCTATGGGGTA +ACCCATAGGACGCTCTAATACGGACATGGCGTGAAGAGTCTATTGAGCTA +GTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCAC +ATACCCTTAATCCAAAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGG +AACCGACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTGTATTGGCTGC +TTATGGTGACAATTAAAGAATTGTTACCATATAGCTATTGGATTGGCCAT +CCAGTGTCAAACAGAGCTATTGTATATCTCTTTGTTGGATTCACACCTCT +CACTCTTGAAACGTTACACACCCTCAATTACATTATACTGCTGAACAC +GAAGCGATGGGCTCCCAGGTCTCCACACAGCGATCCGGCTCGCAT +GAGAATTCCAACTCAGCCACGGAAGGCTCCACTATAAATTACACAACCAT +TAATTACTACAAAGACTCGTATGCTGCCACTGCTGGAAAGCAAAGTCTCA +AACAAGATCCTGACAAGTTTGCGAACCCTGTGAAGGACATCTTTACTGAA +ATGGCAGCGCCCTTAAAGTCTCCCTCTGCTGAAGCATGTGGCTATAGCGA +CCGAGTGGCACAGCTTACCATTGGAAATTCCACCATTACTACACAAGAAG +CAGCAAACATAATAGTTGGGTATGGTGAGTGGCCTTCATACTGCTCTGAT +AATGATGCAACAGCGGTAGACAAACCTACACGGCCTGATGTCTCAGTAAA +TAGATTTTACACGCTAGACACTAAGCTATGGGAGAAATCATCCAAGGGGT +GGTACTGGAAGTTCCCAGATGTACTGACTGAAACCGGAGTTTTTGGTCCA +AATGCACAATTTCACTACTTATACCGTTCAGGGTTCTGCATCCACGTTCA +ATGTAACGCTAGCAAATTTCACCAAGGGGCGCTACTCGTTGCGGTATTGC +CCGAGTATGTCATTGGAACAGTGGCAGGCGGCACAGGCACAGA +GAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCTGATGG +ATTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTC +AGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGT +GCCACCATAATAGTGCCATACATGAACACACTACCTTTTGATTCCGCATT +GAACCACTGTAATTTCGGACTATTGGTGGTGCCTATCAGCCCGCTGGAT +TTCGACCAAGGGGCGACACCGGTAATTCCTATCACTATCACGTTGGCTCC +GATGTGTTCTGAGTTTGCGGGTCTCAGGCAGGCAGTTACGCAGGGTTTTC +CCACTGAATTGAAACCTGGCACTAATCAGTTCTTAACCACGGATGATGGT +GTGTCAGCACCTATATTGCCAAATTTCCACCCCACCCCGTGCATTCACAT +ACCTGGCGAGGTTAGAAACTTACTAGAACTGTGCCAGGTAGAAACCATTT +TAGAAGTCAACAATGTGCCCACCAACGCAACCAGTTTGATGGAAAGGCTA +CGGTTTCCAGTGTCAGCCCAAGCAGGGAAAGGTGAGTTGTGTGCAGTGTT +CAGGGCCGACCCTGGGAGGGATGGTCCTTGGCAATCCACCATGCTAGGCC +AGTTGTGTGGATATTACACCCAATGGTCAGGGTCTTTGGAAGTCACTTTT +ATGTTCACCGGATCCTTTATGGCAACTGGTAAAATGCTTATAGCTTACAC +ACCCCCAGGGGGCCCTTTGCCTAAAGATAGAGCCACAGCTATGCTGGGGA +CGCACGTCATCTGGGACTTTGGCTTGCAATCGTCCGTCACCCTCGTCATA +CCATGGATCAGTAACACTCACTATAGGGCGCATGCTCGAGATGGGGTGTT +TGATTACTACACCACAGGTTTGGTTAGTATATGGTACCAAACAAATTATG +TAGTCCCTATTGGAGCACCTAATACTGCCTATATAATAGCGTTGGCAGCA +GCCCAAAAGAATTTCACTATGAAATTGTGCAAGGACACCAGTGACATTTT +GGAAACGGCCACTATTCAAGGGGACAGAGTGGCAGATGTGATTGAGAGCT +CTATAGGAGATAGTGTGAGTAAGGCCCTCACCCCAGCTTTACCTGCACC +CACAGGCCCAGACACCCAAGTGAGCAGTCATCGCTTAGACACTGGAAA +AGTACCAGCACTTCAAGCCGCCGAAATCGGAGCTTCGTCGAATGCTAGTG +ATGAGAGTATGATTGAGACTCGGTGTGTTCTTAACTCACATAGCACAGCT +GAAACCACCCTTGATAGTTTCTTCAGCAGAGCAGGCTTAGTTGGGGAGAT +AGATCTTCCTCTAAAGGGCACCACCAATCCGAACGGGTATGCCAACTGGG +ACATAGACATAACCGGTTATGCGCAGATGCGCAGAAAAGTGGAACTATTC +ACCTATATGCGCTTTGACGCAGAGTTCACTTTTGTCGCGTGCACACCTAC +CGGAAGGGTCGTTCCACAGCTGCTTCAATACATGTTTGTTCCACCCGGGG +CCCCCAAACCAGACTCCAGAGACTCTTTGGCTTGGCCAACGGCCACGAAC +CCCTCAGTTTTTGTCAAATCATCCGACCCACCAGCACAAGTCTCAGTGCC +ATTTATGTCACCTGCAAGCGCATACCAATGGTTTTATGACGGATACCCTA +CATTTGGAGAGCACAAGCAAGAGAAGGATCTCGAGTATGGGGCATGCCCG +AATAACATGATGGGCACATTCTCAGTGCGGACTGTGGGATCGTCAAAGTC +AGAATATTCCTTAGTCATCAGAATATACATGAGAATGAAGCACGTCAGAG +CGTGGATACCTCGGCCGATGCGCAATCAGAACTATTTGTTCAAATCCAAC +CCAAACTATGCTGGTGATTCCATTAAACCAACTGGTACCAGCCGAA +CGGCAATCACTA +CGCTCGGGAAATTCGGTCAGCAGTCTGGGGCTATTTATGTGGGCAACTTT +AGGGTAGTAAACAGACACCTAGCCACCCATACTGACTGGGCCAACTTGGT +GTGGGAAGACAGCTCTAGAGACCTCCTAGTTTCTTCAACTACCGCTCAAG +GGTGTGACACCATTGCTCGATGTAACTGCCAAACCGGAGTGTATTACTGT +AACTCTCGCAGAAAACACTATCCAGTCAGTTTTTCGAAACCTAGTTTGGT +GTTTGTAGAAGCTAGTGAGTATTATCCAGCTAGATATCAGTCCCATCTTA +TGCTTGCTGAGGGCCATTCAGAACCTGGTGATTGTGGCGGTATTCTTAGA +TGCCAACACGGTGTGGTGGGAATTGTCTCCACTGGCGGAAGTGGCCTTGT +GGGATTTGCTGACGTTAGAGATCTTCTGTGGCTAGATGAGGAAGCGATGG +AGCAGGGGGTATCTGATTACATCAAAGGTCTCGGTCGAGCCTTCGGCACA +GGTTTCACTGACGCAGTGTCTAGGGAAGTGGAAGCGTTGAAGAACCACTT +AATCGGCTCCGAAGGGGCTGTTGAGAAGATCTTGAAGAACTTGGTGAAGC +TAATTTCAGCCTTAGTTATAGTCATCAGAAGTGATTATGATATGGTCACC +CTCACAGCCACACTAGCTCTGATCGGGTGCCACGGGAGTCCTTGGGCGTG +GATCAAATCAAAGACAGCTTCCATACTGGGCATTCCCATGGCACAAAAAC +AGAGTGCCTCATGGCTAAAGAAGTTCAATGACATGGCAAATGCTGCAAAA +GGGCTTGAGTGGATTTTCAACAAGATCAGTAAGTTCATTGACTGGCTTAA +AGAGAAGATCATTCCAGCTGCCAAAGAGAAAGTTGAGTTTTTGAACAACC +TAAAACAGCTCCCCTTGTTGGAGAACCAAGTCTCCAATCTTGAACAGTCT +GCTGCCTCACAAGAAGACTTAGAAGCTATGTTTGGTAATGTGATATATCT +GGCTCACTTTTGCCGCAAATTCCAACCACTCTACGCAACTGAGGCCAAGA +GAGTCTACGCTTTAGAGAAAAGGATGAATAACTACATGCAGTTCAAGAGC +AAACACCGTATTGAACCTGTATGCTTGATCATCAGAGGTTCCCCCGGAAC +GGGCAAATCGCTCGCCACAGGCATTATAGCTAGAGCCATTGCTGACAAGT +ATCGCTCTAGTGTATACTCACTCCCCCCAGACCCAGATCACTTTGATGGG +TATAAGCAACAGGTGGTCGCGGTCATGGATGATCTCTGCCAGAACCCGGA +CGGAAAAGACATGTCCCTATTTTGTCAAATGGTTTCTACAGTAGATTTTG +TACCACCCATGGCATCACTAGAGGAGAAAGGAGTGTCCTTCACCTCTAAG +TTTGTCATTGCATCGACCAATGCTAGTAACATCATAGTCCCCACAGTTTC +AGATTCAGATGCAATTCGCAGGCGATTCTATATGGACTGCGATATAGAAG +TGACAGATTCTTACAAGACAGACCTCGGTCGGCTGGACGCAGGTAGAGCT +GCCAAGCTTTGTACAGAAAATAACACTGCTAATTTTAAGAGATGCAGCCC +ACTGGTGTGTGGTAAGGCTATTCAGCTGAGAGACAGGAAGTCCAAAGTGA +GATATAGCGTCGACACCGTGGTATCGGAACTGATCAGAGAGTACAACAAT +AGATCTGCTATTGGGAATACTATAGAAGCACTCTTTCAAGGACCCCCTAA +ATTCAGGCCTATAAGAATTAGTCTCGAAGAAAAGCCAGCCCCAGATGCCA +TTAGCGATCTCCTCGCTAGTGTAGATAGCGAGGAGGTGCGTCAGTACTGC +AGGGAACAAGGCTGGATCATCCCTGAAACTCCCACCAATGTTGAGCGTCA +CCTCAATAGAGCAGTATTGGTAATGCAGTCCATCGCCACTGTGGTTGCAG +TTGTGTCTCTTGTTTATGTCATTTATAAGCTGTTTGCCGGGTTCCAGGGT +GCTTACTCTGGAGCGCCCAAGCCCATTCTCAAGAAGCCCGTGTTAAGAAC +AGCCACGGTCCAAGGGCCCAGCTTAGACTTCGCCTTGTCTCTTTTGAGGC +GCAACATTAGACAAGCGCAAACTGACCAAGGACACTTCACCATGCTAGGA +GTGCGAGATCGCCTAGCCATCCTGCCGCGCCACTCGCAACCAGGGAAGAC +CATCTGGGTAGAGCATAAATTAATCAATGTACTAGATGCAGTTGAGTTGG +TGGATGAGCAAGGTGTAAACTTGGAACTCACACTGGTAACTTTGGACACC +AATGAAAAATTTAGGGATATCACCAAGTGTATCCCAGAAGTGATCACCGG +GGCGAGTGACGCAACTCTAGTCATCAACACTGAGCACATTCCCTCAATGT +TTGTGCCGGTGGGTGACGTTGTGCAGTACGGTTTCTTGAACCTTAGTGGT +AAACCCACACACAGAACCATGATGTATAACTTCCCCACGAAGCCAGGACA +GTGTGGGGGGGTGGTTACCTCAGTTGGTAAGATCATTGGAATCCACATTG +GCGGGAATGGACGCCAGGCCTTTTGCGCTGGCCTAAAGAGGAGTTATTTT +GCCAGCGAGCAAGGAGAGATCCAGTGGATGAAGCCTAACAGAGAAACCGG +GAGGTTGAATATTAATGGTCCAACCCGAACTAAGCTGGAACCCAGTGTAT +TCCATGATGTGTTCGAGGGCAACAAGGAACCAGCGGTCCTGACTAGTAAG +GACCCCAGACTTGAGGTTGATTTTGAGCAAGCTTTGTTCTCCAAGTATGT +GGGTAACACCCTGCATGAACCTGATGAGTACGTGACACAGGCTGCTCTCC +ACTACGCAAATCAGCTGAAGCAACTGGACATCAACACCAGCAAGATGAGC +ATGGAAGAAGCGTGCTATGGCACAGAATATTTAGAAGCTATAGACTTGCA +CACCAGTGCTGGATACCCTTATAGTGCTTTGGGCATCAAGAAAAGAGACA +TCCTCGACCCAGTTACCAGAGACACCTCCAGGATGAAGTTATATATGGAT +AAGTATGGGTTGGACTTGCCTAATTCCACTTATGTAAAGGATGAGCTTAG +TTCTCTAGATAAGATCAGAAAAGGGGAGTCTCGCCTGATTGAGGCTAGCA +GCTTAAATGATCCTGTCTACCCTAGATTGACTTTTGGACACCTTTATGAA +GTGTTTCACGCCAACCCAGGGACTGTAACAGGATCTGCAGTTGGGTGCAA +CCCTGATGTATTTTGGAGCAAGTTACCAATTTTGTTACCGGGTTCACTCT +TTGCATTTGACTACTCAGGATATGATGCAAGCCTTAGTCCTGTGTGGTTC +AGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGT +GTCACTCATAGAAGGGATCAATCACACCCACCACGTGTACCGAAACAAGA +CATATTGTGTACTTGGTGGAATGCCCTCAGGCTGCTCCGGTACTTCCATT +TTCAATTCCATGATTAACAACATAATCATCAGAACCCTCCTGATTAAAAC +ATTCAAAGGTATAGACTTAGATGAGCTGAAAATGGTAGCTTATGGAGATG +ACGTGTTGGCCAGCTACCCGTTTCCTATTGATTGCTTGGAATGGGGTAAA +ACAGGCAAAGAATATGGGCTGACTATGACTCCTGCTGATAAATCACCTTG +TTTCAATGAGGTTACCTGGGAGAATGCAACCTTCTTAAAACGGGGTTTTC +TACCGGACCATCAGTTCCCTTTTCTGATCCATCCCACTATGCCCATGAGG +GAAATCCATGAGTCCATCCGCTGGACCAAGGACGCGCGCAATACTCAAGA +TCATGTGCGCTCCCTTTGTCTCCTGGCATGGCATAATGGAAAAGAGGAGT +ATGAGAAATTTGTGAGTACAATTAGATCAGTCCCCATTGGAAGGGCTTTA +GCAATTCCAAATTTGGAGAACTTGAGAAGAAATTGGCTCGAGTTATTTTA +AACTTACAGCTCAATGCTGAACCCCACCAGAAATCTGGTCGTGTCAAT +GACTGGTGGGGGTAAATTTGTTATAACCAGAATAGC diff --git a/data/eva71_plus_perfect.fq b/data/eva71_plus_perfect.fq new file mode 100755 index 0000000..63186fc --- /dev/null +++ b/data/eva71_plus_perfect.fq @@ -0,0 +1,2820 @@ +@EVA71_BrCr_U22521-1480 +TCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGTGTCACTCATAGAAGGGATCAATCACACCCACCACGTGTACCGAAACAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1478 +TTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTCAGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGTG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1475 +CCCACGAAGCCAGGACAGTGTGGGGGGGTGGTTACCTCAGTTGGTAAGATCATTGGAATCCACATTGGCGGGAATGGACGCCAGGCCTTTTGCGCTGGCC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1474 +ATCCTGACAAGTTTGCGAACCCTGTGAAGGACATCTTTACTGAAATGGCAGCGCCCTTAAAGTCTCCCTCTGCTGAAGCATGTGGCTATAGCGACCGAGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1473 +GAACAGTGGCAGGCGGCACAGGCACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCTGATGGATTTGAATTACAACATCCATATGTTCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1471 +ACTTCGCCTTGTCTCTTTTGAGGCGCAACATTAGACAAGCGCAAACTGACCAAGGACACTTCACCATGCTAGGAGTGCGAGATCGCCTAGCCATCCTGCC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1468 +CCACTTATGTAAAGGATGAGCTTAGTTCTCTAGATAAGATCAGAAAAGGGGAGTCTCGCCTGATTGAGGCTAGCAGCTTAAATGATCCTGTCTACCCTAG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1467 +TGAGCTTAGTTCTCTAGATAAGATCAGAAAAGGGGAGTCTCGCCTGATTGAGGCTAGCAGCTTAAATGATCCTGTCTACCCTAGATTGACTTTTGGACAC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1462 +ATCCGACCCACCAGCACAAGTCTCAGTGCCATTTATGTCACCTGCAAGCGCATACCAATGGTTTTATGACGGATACCCTACATTTGGAGAGCACAAGCAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1457 +TGACATACCAGTCGCATCTTGATCAAGCACTTCTGTATCCCCGGACCGAGTATCAATAGACTGTGCACACGGTTGAAGGAGAAAACGTCCGTTACCCGGC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1455 +GAACTATTTGTTCAAATCCAACCCAAACTATGCTGGTGATTCCATTAAACCAACTGGTACCAGCCGAACGGCAATCACTACGCTCGGGAAATTCGGTCAG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1448 +CTGGTGTGTGGTAAGGCTATTCAGCTGAGAGACAGGAAGTCCAAAGTGAGATATAGCGTCGACACCGTGGTATCGGAACTGATCAGAGAGTACAACAATA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1446 +TTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTCAGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGTGCCACCA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1443 +ATCCAGTGTCAAACAGAGCTATTGTATATCTCTTTGTTGGATTCACACCTCTCACTCTTGAAACGTTACACACCCTCAATTACATTATACTGCTGAACAC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1440 +AGTGGTAAACCCACACACAGAACCATGATGTATAACTTCCCCACGAAGCCAGGACAGTGTGGGGGGGTGGTTACCTCAGTTGGTAAGATCATTGGAATCC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1439 +ACTCGTTGCGGTATTGCCCGAGTATGTCATTGGAACAGTGGCAGGCGGCACAGGCACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1438 +GCACTAATCAGTTCTTAACCACGGATGATGGTGTGTCAGCACCTATATTGCCAAATTTCCACCCCACCCCGTGCATTCACATACCTGGCGAGGTTAGAAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1436 +TACCGGGTTCACTCTTTGCATTTGACTACTCAGGATATGATGCAAGCCTTAGTCCTGTGTGGTTCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1435 +AGTCTCAGTGCCATTTATGTCACCTGCAAGCGCATACCAATGGTTTTATGACGGATACCCTACATTTGGAGAGCACAAGCAAGAGAAGGATCTCGAGTAT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1427 +AACCAGGGAAGACCATCTGGGTAGAGCATAAATTAATCAATGTACTAGATGCAGTTGAGTTGGTGGATGAGCAAGGTGTAAACTTGGAACTCACACTGGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1426 +ATAAGCAACAGGTGGTCGCGGTCATGGATGATCTCTGCCAGAACCCGGACGGAAAAGACATGTCCCTATTTTGTCAAATGGTTTCTACAGTAGATTTTGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1425 +TGGACACCTTTATGAAGTGTTTCACGCCAACCCAGGGACTGTAACAGGATCTGCAGTTGGGTGCAACCCTGATGTATTTTGGAGCAAGTTACCAATTTTG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1423 +GAAGGGCTTTAGCAATTCCAAATTTGGAGAACTTGAGAAGAAATTGGCTCGAGTTATTTTAAACTTACAGCTCAATGCTGAACCCCACCAGAAATCTGGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1422 +TCGGCTCCGAAGGGGCTGTTGAGAAGATCTTGAAGAACTTGGTGAAGCTAATTTCAGCCTTAGTTATAGTCATCAGAAGTGATTATGATATGGTCACCCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1421 +GTGGCGGTATTCTTAGATGCCAACACGGTGTGGTGGGAATTGTCTCCACTGGCGGAAGTGGCCTTGTGGGATTTGCTGACGTTAGAGATCTTCTGTGGCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1420 +CCAATGCTAGTAACATCATAGTCCCCACAGTTTCAGATTCAGATGCAATTCGCAGGCGATTCTATATGGACTGCGATATAGAAGTGACAGATTCTTACAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1418 +TCACCCCAGCTTTACCTGCACCCACAGGCCCAGACACCCAAGTGAGCAGTCATCGCTTAGACACTGGAAAAGTACCAGCACTTCAAGCCGCCGAAATCGG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1417 +ACCTCCAGGATGAAGTTATATATGGATAAGTATGGGTTGGACTTGCCTAATTCCACTTATGTAAAGGATGAGCTTAGTTCTCTAGATAAGATCAGAAAAG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1416 +CTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTGTATTGGCTGCTTATGGTGACAATTAAAGAATTGTTACCATATAGCTATT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1415 +CGGCCGATGCGCAATCAGAACTATTTGTTCAAATCCAACCCAAACTATGCTGGTGATTCCATTAAACCAACTGGTACCAGCCGAACGGCAATCACTACGC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1413 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+TGAGGTTACCTGGGAGAATGCAACCTTCTTAAAACGGGGTTTTCTACCGGACCATCAGTTCCCTTTTCTGATCCATCCCACTATGCCCATGAGGGAAATC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-75 +TCATACCATGGATCAGTAACACTCACTATAGGGCGCATGCTCGAGATGGGGTGTTTGATTACTACACCACAGGTTTGGTTAGTATATGGTACCAAACAAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-71 +GAAATTGGCTCGAGTTATTTTAAACTTACAGCTCAATGCTGAACCCCACCAGAAATCTGGTCGTGTCAATGACTGGTGGGGGTAAATTTGTTATAACCAG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-70 +TATAGCGACCGAGTGGCACAGCTTACCATTGGAAATTCCACCATTACTACACAAGAAGCAGCAAACATAATAGTTGGGTATGGTGAGTGGCCTTCATACT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-68 +CTAGGAGTGCGAGATCGCCTAGCCATCCTGCCGCGCCACTCGCAACCAGGGAAGACCATCTGGGTAGAGCATAAATTAATCAATGTACTAGATGCAGTTG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-65 +GGGCTTGAGTGGATTTTCAACAAGATCAGTAAGTTCATTGACTGGCTTAAAGAGAAGATCATTCCAGCTGCCAAAGAGAAAGTTGAGTTTTTGAACAACC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-63 +GCCAGCGAGCAAGGAGAGATCCAGTGGATGAAGCCTAACAGAGAAACCGGGAGGTTGAATATTAATGGTCCAACCCGAACTAAGCTGGAACCCAGTGTAT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-61 +ACAGTAGATTTTGTACCACCCATGGCATCACTAGAGGAGAAAGGAGTGTCCTTCACCTCTAAGTTTGTCATTGCATCGACCAATGCTAGTAACATCATAG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-60 +CATCGCTTAGACACTGGAAAAGTACCAGCACTTCAAGCCGCCGAAATCGGAGCTTCGTCGAATGCTAGTGATGAGAGTATGATTGAGACTCGGTGTGTTC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-53 +GGCGCTACTCGTTGCGGTATTGCCCGAGTATGTCATTGGAACAGTGGCAGGCGGCACAGGCACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-51 +ATATGATGCAAGCCTTAGTCCTGTGTGGTTCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGTGTCACTCATAGAAGGGATC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-49 +CAAGTGAGCAGTCATCGCTTAGACACTGGAAAAGTACCAGCACTTCAAGCCGCCGAAATCGGAGCTTCGTCGAATGCTAGTGATGAGAGTATGATTGAGA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-44 +GTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGTGCCACCATAATAGTGCCATACATGAACACACTACCTTTTGATTCCGCATTGAACCACT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-43 +CCGGCGCTGATGGATTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTCAGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-42 +GATAATGATGCAACAGCGGTAGACAAACCTACACGGCCTGATGTCTCAGTAAATAGATTTTACACGCTAGACACTAAGCTATGGGAGAAATCATCCAAGG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-40 +TCAAAGTCAGAATATTCCTTAGTCATCAGAATATACATGAGAATGAAGCACGTCAGAGCGTGGATACCTCGGCCGATGCGCAATCAGAACTATTTGTTCA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-39 +CACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCTGATGGATTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-37 +ACCATTGGAAATTCCACCATTACTACACAAGAAGCAGCAAACATAATAGTTGGGTATGGTGAGTGGCCTTCATACTGCTCTGATAATGATGCAACAGCGG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-34 +TCGTCCGTCACCCTCGTCATACCATGGATCAGTAACACTCACTATAGGGCGCATGCTCGAGATGGGGTGTTTGATTACTACACCACAGGTTTGGTTAGTA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-32 +TCATTCCAGCTGCCAAAGAGAAAGTTGAGTTTTTGAACAACCTAAAACAGCTCCCCTTGTTGGAGAACCAAGTCTCCAATCTTGAACAGTCTGCTGCCTC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-29 +TTGGTGAAGCTAATTTCAGCCTTAGTTATAGTCATCAGAAGTGATTATGATATGGTCACCCTCACAGCCACACTAGCTCTGATCGGGTGCCACGGGAGTC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-26 +ACAAAAACAGAGTGCCTCATGGCTAAAGAAGTTCAATGACATGGCAAATGCTGCAAAAGGGCTTGAGTGGATTTTCAACAAGATCAGTAAGTTCATTGAC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-25 +GGGGTGGTTACCTCAGTTGGTAAGATCATTGGAATCCACATTGGCGGGAATGGACGCCAGGCCTTTTGCGCTGGCCTAAAGAGGAGTTATTTTGCCAGCG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-23 +CAACAAGGAACCAGCGGTCCTGACTAGTAAGGACCCCAGACTTGAGGTTGATTTTGAGCAAGCTTTGTTCTCCAAGTATGTGGGTAACACCCTGCATGAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-22 +AGCCTTAGTCCTGTGTGGTTCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGTGTCACTCATAGAAGGGATCAATCACACCC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-20 +GTGGCTATAGCGACCGAGTGGCACAGCTTACCATTGGAAATTCCACCATTACTACACAAGAAGCAGCAAACATAATAGTTGGGTATGGTGAGTGGCCTTC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-19 +AGGTATAGACTTAGATGAGCTGAAAATGGTAGCTTATGGAGATGACGTGTTGGCCAGCTACCCGTTTCCTATTGATTGCTTGGAATGGGGTAAAACAGGC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-18 +TCCCCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCTATGGGGTAACCCATAGGACGCTCTAATACGGACATGGCGTGAAGAGTCTATTGAGC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-17 +GAACCCCTCAGTTTTTGTCAAATCATCCGACCCACCAGCACAAGTCTCAGTGCCATTTATGTCACCTGCAAGCGCATACCAATGGTTTTATGACGGATAC ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-11 +GTAACATCATAGTCCCCACAGTTTCAGATTCAGATGCAATTCGCAGGCGATTCTATATGGACTGCGATATAGAAGTGACAGATTCTTACAAGACAGACCT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-10 +CCGCTGGACCAAGGACGCGCGCAATACTCAAGATCATGTGCGCTCCCTTTGTCTCCTGGCATGGCATAATGGAAAAGAGGAGTATGAGAAATTTGTGAGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-9 +TCCAGCTGCCAAAGAGAAAGTTGAGTTTTTGAACAACCTAAAACAGCTCCCCTTGTTGGAGAACCAAGTCTCCAATCTTGAACAGTCTGCTGCCTCACAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-6 +GCCATACATGAACACACTACCTTTTGATTCCGCATTGAACCACTGTAATTTCGGACTATTGGTGGTGCCTATCAGCCCGCTGGATTTCGACCAAGGGGCG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-5 +ATGGCGTGAAGAGTCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCACATACCCTTAATCCAAAGGGCAGTGT ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-4 +AGCTTAAATGATCCTGTCTACCCTAGATTGACTTTTGGACACCTTTATGAAGTGTTTCACGCCAACCCAGGGACTGTAACAGGATCTGCAGTTGGGTGCA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ diff --git a/tests/__init__.py b/tests/__init__.py new file mode 100644 index 0000000..e69de29 diff --git a/tests/context.py b/tests/context.py new file mode 100644 index 0000000..649f009 --- /dev/null +++ b/tests/context.py @@ -0,0 +1,9 @@ +import os +import sys +# Path of debruijn package +#sys.path.insert(0, os.path.abspath(os.path.join(os.path.dirname(__file__), +# '..'))) +# Path for core +sys.path.insert(0, os.path.abspath(os.path.join(os.path.dirname(__file__), + '../debruijn'))) +import debruijn diff --git a/tests/kmer.pck b/tests/kmer.pck new file mode 100644 index 0000000..d035ad7 --- /dev/null +++ b/tests/kmer.pck @@ -0,0 +1,14 @@ +(dp0 +S'GAG' +p1 +I1 +sS'CAG' +p2 +I1 +sS'AGA' +p3 +I2 +sS'TCA' +p4 +I1 +s. \ No newline at end of file diff --git a/tests/test_build.fq b/tests/test_build.fq new file mode 100644 index 0000000..9057446 --- /dev/null +++ b/tests/test_build.fq @@ -0,0 +1,4 @@ +@EVA71_BrCr_U22521-1480 +TCAGAGA ++ +JJJJJJJ diff --git a/tests/test_construction_debruijn.py b/tests/test_construction_debruijn.py new file mode 100644 index 0000000..e30a8ec --- /dev/null +++ b/tests/test_construction_debruijn.py @@ -0,0 +1,51 @@ +"""Tests for graph build""" +import pytest +import os +import networkx as nx +import pickle +from .context import debruijn +from debruijn import read_fastq +from debruijn import cut_kmer +from debruijn import build_kmer_dict +from debruijn import build_graph + + +def test_read_fastq(): + """Test fastq reading""" + fastq_reader = read_fastq(os.path.abspath(os.path.join(os.path.dirname(__file__), "test_two_reads.fq"))) + assert next(fastq_reader) == "TCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGTGTCACTCATAGAAGGGATCAATCACACCCACCACGTGTACCGAAACAA" + assert next(fastq_reader) == "TTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTCAGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGTG" + + +def test_cut_kmer(): + """test Kmer cut""" + kmer_reader = cut_kmer("TCAGA", 3) + assert next(kmer_reader) == "TCA" + assert next(kmer_reader) == "CAG" + assert next(kmer_reader) == "AGA" + + +def test_build_kmer_dict(): + kmer_dict = build_kmer_dict(os.path.abspath(os.path.join(os.path.dirname(__file__), "test_build.fq")), 3) + assert(len(kmer_dict.keys()) == 4) + assert "TCA" in kmer_dict + assert "CAG" in kmer_dict + assert "AGA" in kmer_dict + assert "GAG" in kmer_dict + assert kmer_dict["AGA"] == 2 + +def test_build_graph(): + file = open(os.path.abspath(os.path.join(os.path.dirname(__file__), "kmer.pck")),'rb') + kmer_dict = pickle.load(file) + graph = build_graph(kmer_dict) + #TCAGAGA + #TCA TC CA + #CAG CA AG + #AGA AG GA + #GAG GA AG + #AGA AG GA + assert graph.number_of_nodes() == 4 + assert graph.number_of_edges() == 4 + assert "AG" in graph + assert "GA" in graph + assert graph.edges["AG", "GA"]['weight'] == 2 \ No newline at end of file diff --git a/tests/test_two_reads.fq b/tests/test_two_reads.fq new file mode 100644 index 0000000..93c54a8 --- /dev/null +++ b/tests/test_two_reads.fq @@ -0,0 +1,8 @@ +@EVA71_BrCr_U22521-1480 +TCAGAGCTCTAGAGTTGGTTCTGAGAGAGATCGGTTACTCGGAGGAGGCTGTGTCACTCATAGAAGGGATCAATCACACCCACCACGTGTACCGAAACAA ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ +@EVA71_BrCr_U22521-1478 +TTTGAATTACAACATCCATATGTTCTTGATGCTGGAATTCCAATATCTCAGTTGACAGTGTGCCCTCACCAGTGGATCAATTTACGAACCAACAATTGTG ++ +JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ