|
@@ -28,12 +28,12 @@ soft/megahit --k-list 21 --mem-flag 0 -1 EchE_R1.fastq.gz -2 EchE_R2.fastq.gz -o
|
28
|
28
|
|
29
|
29
|
# Question 4
|
30
|
30
|
|
31
|
|
-soft/prodigal -i contigs/final.contigs.fa -d genes.fna
|
|
31
|
+soft/prodigal -i contigs/final.contigs.fa -d results/genes.fna
|
32
|
32
|
|
33
|
33
|
# Question 5
|
34
|
34
|
|
35
|
|
-sed "s:>:*\n>:g" genes.fna | sed -n "/partial=00/,/*/p"|grep -v "*" > genes_full.fna
|
|
35
|
+sed "s:>:*\n>:g" results/genes.fna | sed -n "/partial=00/,/*/p"|grep -v "*" > results/genes_full.fna
|
36
|
36
|
|
37
|
37
|
# Question 6
|
38
|
38
|
|
39
|
|
-
|
|
39
|
+blastn -query results/genes_full.fna -db databases/resfinder.fna -perc_identity 0.8 -qcov_hsp_perc 0.8 -evalue 1e-3 -outfmt 6 -out results/annoted_genes.dat
|